Description
Boster’s human EGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for EGF has been precoated onto 96-well plates. Standards(E.coli, N971-R1023) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human EGF amount of sample captured in plate.
Synonyms: AI790464|Beta urogastrone|Epidermal growth factor|HOMG4|OTTHUMP00000219721|OTTHUMP00000219722|Pro epidermal growth factor|Pro epidermal growth factor precursor (EGF)|URG|Urogastrone
Species: Human EGF
Assay: 4.7pg/ml-300pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human EGF
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: E.coli, N971-R1023
Gene name: Pro-epidermal growth factor / epidermal growth factor
Protein name: Pro-epidermal growth factor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|11|76|122|10|81|131
Standard deviation|0.71|4.25|9.15|0.82|5.42|12.31
CV(%)|6.5%|5.6%|7.5%|8.2%|6.7%|9.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 11 | 11 | 11 | 12 | 11 | 0.43 | 3.9% |
| Sample 2 | 76 | 76 | 72 | 68 | 73 | 3.31 | 4.5% |
| Sample 3 | 122 | 118 | 128 | 133 | 125 | 5.71 | 4.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0325-CAP|96-well plate precoated with anti- human EGF antibody|1
EK0325-ST|Lyophilized recombinant human EGF standard|1ng/tube×2
EK0325-DA|Biotinylated anti- human EGF antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
