Description
Boster’s human DKK1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DKK1 has been precoated onto 96-well plates. Standards(sf21,T32-H266) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DKK1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DKK1 amount of sample captured in plate.
Synonyms: Dickkopf 1|_x000D_Dickkopf homolog 1|_x000D_Dickkopf related protein 1|_x000D__x000D__x000D_DKK 1|_x000D_hDkk 1|_x000D_hDkk1|_x000D_mDkk1|_x000D_SK
Species: Human DKK-1
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Oligomeric forms of natural and recombinant human DKK1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: sf21, T32-H266
Gene name: Dickkopf-related protein 1 / dickkopf WNT signaling pathway inhibitor 1
Protein name: Dickkopf-related protein 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|108|660|1140|103|594|1134
Standard deviation|5.72|47.52|66.12|6.59|43.36|82.78
CV(%)|5.3%|7.2%|5.8%|6.4%|7.3%|7.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 108 | 125 | 119 | 115 | 116 | 6.17 | 5.3% |
| Sample 2 | 660 | 564 | 616 | 629 | 617 | 34.65 | 5.6% |
| Sample 3 | 1140 | 1087 | 1155 | 1110 | 1123 | 26.35 | 2.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0867-CAP|96-well plate precoated with anti- human DKK1 antibody|1
EK0867-ST|Lyophilized recombinant human DKK1 standard|10ng/tube×2
EK0867-DA|Biotinylated anti- human DKK1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
