Description
Boster’s human Diablo ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Diablo has been precoated onto 96-well plates. Standards(E.coli, A56-D239) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Diablo is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Diablo amount of sample captured in plate.
Synonyms: DFNA64|DIABLO|diablo homolog, mitochondrial|diablo, IAP-binding mitochondrial protein|DIABLO-S|direct IAP-binding protein with low pI|mitochondrial Smac protein|second mitochondria-derived activator of caspase|SMAC3
Species: Human Diablo
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Diablo
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, A56-D239
Gene name: Diablo homolog, mitochondrial / diablo, IAP-binding mitochondrial protein
Protein name: Diablo homolog, mitochondrial
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|54|221|865|53|229|932
Standard deviation|4.05|17.01|36.33|4.61|20.15|48.46
CV(%)|7.5%|7.7%|4.2%|8.7%|8.8%|5.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 54 | 54 | 55 | 55 | 54 | 0.5 | 0.9% |
| Sample 2 | 221 | 218 | 222 | 235 | 224 | 6.51 | 2.9% |
| Sample 3 | 865 | 888 | 885 | 882 | 880 | 8.91 | 1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0838-CAP|96-well plate precoated with anti- human Diablo antibody|1
EK0838-ST|Lyophilized recombinant human Diablo standard|10ng/tube×2
EK0838-DA|Biotinylated anti- human Diablo antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
