Description
Boster’s human DCR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DCR3 has been precoated onto 96-well plates. Standards(sf21, V24 – H300) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DCR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DCR3 amount of sample captured in plate.
Synonyms: DcR3|Decoy Receptor 3|Decoy receptor for Fas ligand|DJ583P15.1.1|M68|M68E|OTTHUMP00000031583|TNF6B_HUMAN|TNFRSF6B|TR6|Tumor necrosis factor receptor superfamily member 6B|Tumor necrosis factor receptor superfamily member 6b decoy|Tumor necrosis factor receptor superfamily member 6b decoy|UNQ186/PRO212
Species: Human DCR3
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human DCR3
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: sf21, V24-H300
Gene name: Tumor necrosis factor receptor superfamily member 6B / tumor necrosis factor receptor superfamily, member 6b, decoy
Protein name: Tumor necrosis factor receptor superfamily member 6B
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|272|1644|5011|272|1715|5192
Standard deviation|12.24|124.94|330.72|14.96|145.77|441.32
CV(%)|4.5%|7.6%|6.6%|5.5%|8.5%|8.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 272 | 246 | 272 | 280 | 267 | 12.83 | 4.8% |
| Sample 2 | 1644 | 1634 | 1754 | 1881 | 1728 | 99.97 | 5.7% |
| Sample 3 | 5011 | 5645 | 5121 | 5650 | 5356 | 293.34 | 5.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0748-CAP|96-well plate precoated with anti- human DCR3 antibody|1
EK0748-ST|Lyophilized recombinant human DCR3 standard|10ng/tube×2
EK0748-DA|Biotinylated anti- human DCR3 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
