Description
Boster’s human CXCL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL4 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: E32-S101) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL4 amount of sample captured in plate.
Synonyms: C X C motif chemokine 4|C-X-C motif chemokine 4|Chemokine (C X C motif) ligand 4|Chemokine (CXC motif) ligand 4|Chemokine, CXC motif, ligand 4|CXCL 4|CXCL4|Iroplact|MGC138298|Oncostatin A|Oncostatin-A|OncostatinA|PF 4|PF-4|PF4|Pf4a|Platelet factor 4|PLF4_HUMAN| RATPF4A|SCYB 4|SCYB4|short form|Small inducible cytokine subfamily B, member 4|Small inducible cytokine subfamily member 4
Species: human CXCL4
Assay: 156pg/ml-10000pg/ml
Sensitivy: <15pg/ml Specifity: Natural and recombinant human CXCL4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, E32-S101
Gene name: Platelet factor 4 / platelet factor 4
Protein name: Platelet factor 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|355|1700|4798|337|1547|5118
Standard deviation|24.85|79.9|249.49|24.26|91.27|348.02
CV(%)|7%|4.7%|5.2%|7.2%|5.9%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 355 | 302 | 353 | 316 | 331 | 23.04 | 6.9% |
| Sample 2 | 1700 | 1790 | 1823 | 1661 | 1743 | 65.53 | 3.7% |
| Sample 3 | 4798 | 4682 | 4616 | 4446 | 4635 | 127.33 | 2.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0726-CAP|96-well plate precoated with anti- human CXCL4 antibody|1
EK0726-ST|Lyophilized recombinant human CXCL4 standard|10ng/tube×2
EK0726-DA|Biotinylated anti- human CXCL4 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
