Description
Boster’s human Corin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Corin has been precoated onto 96-well plates. Standards(NSO, S67-N1042) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Corin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Corin amount of sample captured in plate.
Synonyms: activated protease fragment|ATC 2|ATC2|Atrial natriuretic peptide-converting enzyme|Atrial natriuteric peptide converting enzyme|Corin|Corin serine peptidase|CORIN_HUMAN|CRN|Heart specific serine proteinase|Heart specific serine proteinase ATC2|Heart-specific serine proteinase ATC2|Lrp 4|Lrp4|MGC119742|Pro ANP convertase|Pro ANP converting enzyme|Pro-ANP-converting enzyme|TMPRSS 10|TMPRSS10|Transmembrane protease serine 10
Species: human Corin
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Corin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, S67-N1042
Gene name: Atrial natriuretic peptide-converting enzyme / corin, serine peptidase
Protein name: Atrial natriuretic peptide-converting enzyme
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|84|570|1910|81|544|1744
Standard deviation|5.46|39.9|95.5|5.58|42.43|102.89
CV(%)|6.5%|7%|5%|6.9%|7.8%|5.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 84 | 80 | 92 | 87 | 85 | 4.38 | 5.1% |
| Sample 2 | 570 | 521 | 489 | 573 | 538 | 35.13 | 6.5% |
| Sample 3 | 1910 | 1971 | 2154 | 1946 | 1995 | 94.18 | 4.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1283-CAP|96-well plate precoated with anti- human Corin antibody|1
EK1283-ST|Lyophilized recombinant human Corin standard|4ng/tube×2
EK1283-DA|Biotinylated anti- human Corin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
