Description
Boster’s human Chitinase 3-like 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Chitinase 3-like 1 has been precoated onto 96-well plates. Standards(NSO, Y22-T383) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Chitinase 3-like 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Chitinase 3-like 1 amount of sample captured in plate.
Synonyms: 39 kDa synovial protein|ASRT7|Cartilage glycoprotein 39|CGP-39|CGP39|CH3L1_HUMAN|CHI3L1|chitinase 3 like 1 (cartilage glycoprotein 39)|chitinase 3 like 1|Chitinase 3 like protein 1 precursor|chitinase|Chitinase-3-like protein 1|GP 39|GP-39|GP39|HC gp39|HCGP 3P|hCGP-39|HCgp39|YKL 40|YKL-40|YKL40|YYL 40
Species: Human Chitinase 3-like 1
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Chitinase 3-like 1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, Y22-T383
Gene name: Chitinase-3-like protein 1 / chitinase 3-like 1 (cartilage glycoprotein-39)
Protein name: Chitinase-3-like protein 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|90|671|1314|87|650|1207
Standard deviation|4.95|27.51|69.64|6.17|38.35|80.86
CV(%)|5.5%|4.1%|5.3%|7.1%|5.9%|6.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 90 | 84 | 86 | 89 | 87 | 2.38 | 2.7% |
| Sample 2 | 671 | 639 | 681 | 646 | 659 | 17.29 | 2.6% |
| Sample 3 | 1314 | 1141 | 1343 | 1237 | 1258 | 78.24 | 6.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0974-CAP|96-well plate precoated with anti- human Chitinase 3-like 1 antibody|1
EK0974-ST|Lyophilized recombinant human Chitinase 3-like 1 standard|10ng/tube×2
EK0974-DA|Biotinylated anti- human Chitinase 3-like 1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
