Description
Boster’s human CD73 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from sheep specific for CD73 has been precoated onto 96-well plates. Standards(CHO, W27-K547) and test samples are added to the wells, a biotinylated detection polyclonal antibody from sheep specific for CD73 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD73 amount of sample captured in plate.
Synonyms: 5′ NT|5′ nucleotidase (CD73)|5′ nucleotidase precursor|5′ nucleotidase, ecto|5′ nucleotidase, ecto (CD73)|5′-NT|5′-nucleotidase| 5NTD_HUMAN|CD73|CD73 antigen|E5NT|Ecto 5′ nucleotidase|Ecto-5′-nucleotidase|eN|eNT|NT|NT5|NT5E|NTE|Purine 5 Prime Nucleotidase
Species: human CD73
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD73
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from sheep|a biotinylated polyclonal antibody from sheep
Anticoagulant: heparin or EDTA
Immunogen: CHO,W27-K547
Gene name: 5′-nucleotidase / 5′-nucleotidase, ecto (CD73)
Protein name: 5′-nucleotidase
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|230|1941|5361|222|1912|4929
Standard deviation|15.41|79.58|385.99|18.64|101.33|359.81
CV(%)|6.7%|4.1%|7.2%|8.4%|5.3%|7.3%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 230 | 235 | 243 | 260 | 242 | 11.37 | 4.6% |
| Sample 2 | 1941 | 1870 | 1830 | 1891 | 1883 | 40.01 | 2.1% |
| Sample 3 | 5361 | 4741 | 5455 | 5425 | 5245 | 293.24 | 5.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1422-CAP|96-well plate precoated with anti- human CD73 antibody|1
EK1422-ST|Lyophilized recombinant human CD73 standard|10ng/tube×2
EK1422-DA|Biotinylated anti- human CD73 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
