Description
Boster’s human CD36 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD36 has been precoated onto 96-well plates. Standards(sf21, G30-N439) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD36 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD36 amount of sample captured in plate.
Synonyms: CD36 antigen|CD36 antigen (collagen type I receptor thrombospondin receptor)|CD36 molecule|CD36 molecule (thrombospondin receptor)|CHDS7|Cluster determinant 36|FAT|Fatty acid translocase|Glycoprotein IIIb|GP3B|GP4|GPIIIB|GPIV|Leukocyte differentiation antigen CD36|PAS 4 protein|PAS IV|PASIV|Platelet collagen receptor|Platelet glycoprotein 4|Platelet glycoprotein IV|SCARB3|Scavenger receptor class B member 3|Thrombospondin receptor
Species: Human CD36
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD36
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: sf21, G30-N439
Gene name: Platelet glycoprotein 4 / CD36 molecule (thrombospondin receptor)
Protein name: Platelet glycoprotein 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|329|1274|5223|298|1195|5251
Standard deviation|23.03|76.44|360.38|21.45|81.26|456.83
CV(%)|7%|6%|6.9%|7.2%|6.8%|8.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 329 | 358 | 360 | 320 | 341 | 17.55 | 5.1% |
| Sample 2 | 1274 | 1304 | 1194 | 1283 | 1263 | 41.71 | 3.3% |
| Sample 3 | 5223 | 5923 | 6080 | 5774 | 5750 | 322.92 | 5.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0700-CAP|96-well plate precoated with anti- human CD36 antibody|1
EK0700-ST|Lyophilized recombinant human CD36 standard|10ng/tube×2
EK0700-DA|Biotinylated anti- human CD36 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
