Description
Boster’s human CD320 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD320 has been precoated onto 96-well plates. Standards(NSO, S36-Y229) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD320 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD320 amount of sample captured in plate.
Synonyms: 8D6|8D6 antigen|8D6A|CD320|CD320 antigen|CD320_HUMAN|FDC-signaling molecule 8D6|FDC-SM-8D6|TCblR|Transcobalamin receptor
Species: human CD320
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD320
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, S36-Y229
Gene name: CD320 antigen / CD320 molecule
Protein name: CD320 antigen
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|293|1266|4609|322|1142|5029
Standard deviation|14.65|72.16|271.93|17.06|81.08|326.88
CV(%)|5%|5.7%|5.9%|5.3%|7.1%|6.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 293 | 300 | 313 | 285 | 297 | 10.28 | 3.4% |
| Sample 2 | 1266 | 1315 | 1451 | 1426 | 1364 | 76.51 | 5.6% |
| Sample 3 | 4609 | 4247 | 4591 | 4105 | 4388 | 217.95 | 4.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1255-CAP|96-well plate precoated with anti- human CD320 antibody|1
EK1255-ST|Lyophilized recombinant human CD320 standard|10ng/tube×2
EK1255-DA|Biotinylated anti- human CD320 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
