Description
Boster’s human CD32 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD32 has been precoated onto 96-well plates. Standards(NSO, A46-P217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD32 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD32 amount of sample captured in plate.
Synonyms: CD 32|CD32|CD32 antigen|CD32A|CD32B|CD32C|CDw32|Fc fragment of IgG low affinity II receptor for (CD32)|Fc fragment of IgG low affinity IIa receptor|Fc fragment of IgG low affinity IIa receptor for (CD32)|Fc fragment of IgG low affinity IIb receptor|Fc fragment of IgG low affinity IIb receptor for (CD32)|Fc fragment of IgG low affinity IIc receptor|Fc fragment of IgG low affinity IIc receptor for (CD32)|Fc gamma receptor IIB|Fc gamma RII a|Fc gamma RII b|Fc gamma RII c|Fc gamma RIIa|Fc gamma RIIb |Fc gamma RIIc|Fc receptor, IgG, low affinity IIb|Fc-gamma RII-a|Fc-gamma RII-b|Fc-gamma RII-c|Fc-gamma-RIIa|FC-gamma-RIIB|Fc-GAMMA-RIIC|Fc[g]RII|FCG 2|FCG2|FCG2A_HUMAN|FcgammaRIIC|FCGR 2|FcGR|FCGR2|FCGR2A|FCGR2A1|FCGR2B|FCGR2C|Fcgr3|Fcgr3a|FcgRII|Fcr-2|Fcr-3|FcRII a|FCRII|FcRII b|FcRII c|FcRII-a|FcRIIC|IGFR 2|IGFR2|IgG Fc receptor II a|IgG Fc receptor II b|IgG Fc receptor II beta|IgG Fc receptor II c|IgG Fc receptor II-a|IgG Fc receptor II-b|IgG Fc receptor II-c|Immunoglobulin G Fc receptor II|Low affinity|immunoglobulin gamma Fc region receptor II a|Low affinity immunoglobulin gamma Fc region receptor II|Low affinity immunoglobulin gamma Fc region receptor II b|Low affinity immunoglobulin gamma Fc region receptor II c|Low affinity immunoglobulin gamma Fc region receptor II-a|Low affinity immunoglobulin gamma Fc region receptor II-b|Low affinity immunoglobulin gamma Fc region receptor II-c|Ly-17|Ly-m20|LyM-1|Lymphocyte antigen 17|MGC23887|MGC30032
Species: human CD32
Assay: 46.9pg/ml-3000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD32
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, A46-P217
Gene name: Low affinity immunoglobulin gamma Fc region receptor II-b / Fc fragment of IgG, low affinity IIb, receptor (CD32)
Protein name: Low affinity immunoglobulin gamma Fc region receptor II-b
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|122|404|1372|133|365|1478
Standard deviation|8.05|19.79|97.41|9.7|22.26|130.06
CV(%)|6.6%|4.9%|7.1%|7.3%|6.1%|8.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 122 | 125 | 105 | 106 | 114 | 9.06 | 7.9% |
| Sample 2 | 404 | 361 | 357 | 357 | 369 | 19.84 | 5.3% |
| Sample 3 | 1372 | 1213 | 1353 | 1369 | 1326 | 66.06 | 4.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1312-CAP|96-well plate precoated with anti- human CD32 antibody|1
EK1312-ST|Lyophilized recombinant human CD32 standard|10ng/tube×2
EK1312-DA|Biotinylated anti- human CD32 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
