Description
Boster’s human CD26 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD26 has been precoated onto 96-well plates. Standards(NSO, D34-P766) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD26 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD26 amount of sample captured in plate.
Synonyms: ADABP|ADCP-2|ADCP2|Adenosine deaminase complexing protein 2|CD26|Dipeptidyl peptidase 4|Dipeptidyl peptidase 4 soluble form|Dipeptidyl peptidase IV|Dipeptidyl peptidase IV membrane form|Dipeptidyl peptidase IV soluble form|Dipeptidylpeptidase 4|DPP 4|DPP IV|DPP4|DPP4_HUMAN|DPPIV|T-cell activation antigen CD26|TP103
Species: Human CD26
Assay: 312pg/ml-20000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD26
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, D34-P766
Gene name: Dipeptidyl peptidase 4 / dipeptidyl-peptidase 4
Protein name: Dipeptidyl peptidase 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|590|3074|9263|536|3241|9182
Standard deviation|26.55|196.73|574.3|26.26|269|661.1
CV(%)|4.5%|6.4%|6.2%|4.9%|8.3%|7.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 590 | 523 | 573 | 598 | 571 | 29.14 | 5.1% |
| Sample 2 | 3074 | 2972 | 3152 | 2772 | 2992 | 142.41 | 4.7% |
| Sample 3 | 9263 | 9581 | 9698 | 9711 | 9563 | 180.59 | 1.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0696-CAP|96-well plate precoated with anti- human CD26 antibody|1
EK0696-ST|Lyophilized recombinant human CD26 standard|20ng/tubex2
EK0696-DA|Biotinylated anti- human CD26 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
