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Human CD163 / M130 PicoKine™ Fast ELISA Kit

Brand:
Boster Bio
Storage:

The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detect Human CD163 with <150pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CD163 tissue specificity: Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform The Fast version of Picokine ELISA kits, assay takes less than 1.5 hours. Detected in the blood. .   

 

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SKU: FEK0715 Category:

Description

Boster’s human CD163 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD163 has been precoated onto 96-well plates. Standards(NSO, G41-S1045) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD163 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD163 amount of sample captured in plate.

Synonyms: Scavenger receptor cysteine-rich type 1 protein M130;Hemoglobin scavenger receptor;CD163;Soluble CD163;sCD163;CD163;M130;

Species: Human CD163

Assay: 1.56ng/ml-100ng/ml

Sensitivy: <150pg/ml Specifity: Natural and recombinant human CD163

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat

Anticoagulant: heparin

Immunogen: Expression system for standard: NSO; Immunogen sequence: G41-S1045

Gene name: Scavenger receptor cysteine-rich type 1 protein M130 / Scavenger receptor cysteine-rich type 1 protein M130

Protein name: Scavenger receptor cysteine-rich type 1 protein M130

Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 3520 11456 44674 3368 11294 48653
Standard deviation 144.32 526.97 303195.343 195.34 632.46 3357.05
CV(%) 4.1% 4.6% 6.8% 5.8% 5.6% 6.9%

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 3520 3403 3222 3050 3298 178.59 5.4%
Sample 2 11456 11119 13238 12078 11972 807.44 6.7%
Sample 3 44674 42932 42302 44756 43666 1072.77 2.4%

*number of samples for each test n=16.

100ng/tube

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