Description
Boster’s human CCL8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CCL8 has been precoated onto 96-well plates. Standards(E.coli, Q24-P99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL8 amount of sample captured in plate.
Synonyms: C C motif chemokine 8|CCL 8|Ccl8|CCL8_HUMAN|Chemokine (C C motif) ligand 8|Chemokine ligand 8|HC14|MCP 2|MCP-2|MCP-2(6-76)|Monocyte chemoattractant protein 2|Monocyte chemotactic protein 2|SCYA10|SCYA8|Small inducible cytokine A8|Small inducible cytokine subfamily A (Cys Cys) member 8|Small-inducible cytokine A8
Species: Human CCL8
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human CCL8
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, Q24-P99
Gene name: C-C motif chemokine 8 / chemokine (C-C motif) ligand 8
Protein name: C-C motif chemokine 8
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|35|152|451|36|154|486
Standard deviation|2.76|9.27|19.84|3.13|11.24|27.7
CV(%)|7.9%|6.1%|4.4%|8.7%|7.3%|5.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 35 | 36 | 34 | 30 | 33 | 2.27 | 6.8% |
| Sample 2 | 152 | 155 | 149 | 171 | 156 | 8.49 | 5.4% |
| Sample 3 | 451 | 459 | 443 | 431 | 446 | 10.34 | 2.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0442-CAP|96-well plate precoated with anti- human CCL8 antibody|1
EK0442-ST|Lyophilized recombinant human CCL8 standard|10ng/tubex2
EK0442-DA|Biotinylated anti- human CCL8 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
