Description
Boster’s human CCL25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL25 has been precoated onto 96-well plates. Standards(E.coli, Q24-L150) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL25 amount of sample captured in plate.
Synonyms: CCL25 | Chemokine TECK | O15444 | SCY A25 | SCYA25 | SCYA25 | TECK | Small inducible cytokine A25 | Small-inducible cytokine A25 | TECKvar | Thymus-expressed chemokine
Species: human CCL25
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CCL25
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, Q24-L150
Gene name: C-C motif chemokine 25 / chemokine (C-C motif) ligand 25
Protein name: C-C motif chemokine 25
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|181|632|1287|190|596|1194
Standard deviation|12.85|36.02|101.67|15.01|35.76|100.29
CV(%)|7.1%|5.7%|7.9%|7.9%|6%|8.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 181 | 188 | 196 | 190 | 188 | 5.35 | 2.8% |
| Sample 2 | 632 | 606 | 618 | 625 | 620 | 9.6 | 1.5% |
| Sample 3 | 1287 | 1247 | 1348 | 1315 | 1299 | 37.09 | 2.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1145-CAP|96-well plate precoated with anti- human CCL25 antibody|1
EK1145-ST|Lyophilized recombinant human CCL25 standard|10ng/tube×2
EK1145-DA|Biotinylated anti- human CCL25 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
