Description
Boster’s human CCL19 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL19 has been precoated onto 96-well plates. Standards(E.coli, G22-S98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL19 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL19 amount of sample captured in plate.
Synonyms: Beta chemokine exodus 3|Beta-chemokine exodus-3|C C chemokine ligand 19|C-C motif chemokine 19|CC chemokine ligand 19|CCL19|CCL19_HUMAN|Chemokine (C C motif) ligand 19|Chemokine (CC motif) ligand 19|Chemokine CC Motif Ligand 19|CK beta 11|CK beta-11|CKb11|EBI1 ligand chemokine|ELC|Epstein-Barr virus-induced molecule 1 ligand chemokine|Exodus 3|Macrophage inflammatory protein 3 beta|MGC34433|MIP 3 beta|MIP-3-beta|MIP-3B|MIP3 beta|MIP3B|OTTHUMP00000000531|SCYA19|Small inducible cytokine A19|Small-inducible cytokine A19
Species: Human CCL19
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CCL19
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, G22-S98
Gene name: C-C motif chemokine 19 / chemokine (C-C motif) ligand 19
Protein name: C-C motif chemokine 19
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|29|234|421|27|213|398
Standard deviation|1.39|15.67|26.94|1.35|15.97|32.23
CV(%)|4.8%|6.7%|6.4%|5%|7.5%|8.1%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 30 | 31 | 29 | 29 | 0.82 | 2.8% |
| Sample 2 | 234 | 255 | 249 | 222 | 240 | 12.9 | 5.3% |
| Sample 3 | 421 | 456 | 425 | 465 | 441 | 19.07 | 4.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0456-CAP|96-well plate precoated with anti- human CCL19 antibody|1
EK0456-ST|Lyophilized recombinant human CCL19 standard|10ng/tubex2
EK0456-DA|Biotinylated anti- human CCL19 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
