Description
Boster’s human CCL17 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL17 has been precoated onto 96-well plates. Standards(E.coli, A24-S94) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL17 amount of sample captured in plate.
Synonyms: A 152E5.3|ABCD 2|C C motif chemokine 17|C-C motif chemokine 17|CC chemokine TARC|CCL 17|CCL17|CCL17_HUMAN|Chemokine (C C motif) ligand 17|Chemokine ligand 17|MGC138271|MGC138273|SCYA17|Small inducible cytokine A17|Small inducible cytokine subfamily A (Cys Cys) member 17|Small inducible cytokine subfamily A member 17|Small-inducible cytokine A17|T cell directed CC chemokine|Thymus and activation regulated chemokine|Thymus and activation-regulated chemokine
Species: Human CCL17
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <1pg/ml Specifity: Natural and recombinant human CCL17
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: E.coli, A24-S94
Gene name: C-C motif chemokine 17 / chemokine (C-C motif) ligand 17
Protein name: C-C motif chemokine 17
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|62|251|939|56|234|931
Standard deviation|2.6|10.79|53.52|2.68|12.16|62.37
CV(%)|4.2%|4.3%|5.7%|4.8%|5.2%|6.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 62 | 58 | 60 | 57 | 59 | 1.92 | 3.2% |
| Sample 2 | 251 | 287 | 247 | 277 | 265 | 16.93 | 6.3% |
| Sample 3 | 939 | 986 | 993 | 986 | 976 | 21.55 | 2.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0684-CAP|96-well plate precoated with anti- human CCL17 antibody|1
EK0684-ST|Lyophilized recombinant human CCL17 standard|10ng/tubex2
EK0684-DA|Biotinylated anti- human CCL17 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
