Description
Boster’s human CANT1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CANT1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: R63-I401) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CANT1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CANT1 amount of sample captured in plate.
Synonyms: Soluble calcium-activated nucleotidase 1; SCAN-1; Apyrase homolog; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107; CANT1; SHAPY
Species: Human CANT1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant human CANT1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated monoclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO, R63-I401
Gene name: Soluble calcium-activated nucleotidase 1 / calcium activated nucleotidase 1
Protein name: Soluble calcium-activated nucleotidase 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|319|1085|5570|313|1102|5558
Standard deviation|16.26|44.48|239.51|17.21|50.69|316.8
CV(%)|5.1%|4.1%|4.3%|5.5%|4.6%|5.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 319 | 341 | 313 | 295 | 317 | 16.43 | 5.1% |
| Sample 2 | 1085 | 1189 | 1030 | 1018 | 1080 | 67.54 | 6.2% |
| Sample 3 | 5570 | 5244 | 5973 | 5762 | 5637 | 268.07 | 4.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK2109-CAP|96-well plate precoated with anti- human CANT1 antibody|1
EK2109-ST|Lyophilized recombinant human CANT1 standard|10ng/tube×2
EK2109-DA|Biotinylated anti- human CANT1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
