Human B7-1/CD80 PicoKine ELISA Kit

Brand:
Boster Bio
Storage:

For quantitative detection of human CD80 in cell culture supernates and serum.   

 

Available on backorder

SKU: EK0707 Category:

Description

Boster’s human CD80 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD80 has been precoated onto 96-well plates. Standards(NSO, V35-N242) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for CD80 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD80 amount of sample captured in plate.


Synonyms: Activation B7-1 antigen|B7|BB1|CD80|CD80_HUMAN|CTLA-4 counter-receptor B7.1|T-lymphocyte activation antigen CD80

Species: Human B7-1

Assay: 62.5pg/ml-4000pg/ml

Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD80

Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

Antibodies: a monoclonal antibody from mouse|a biotinylated monoclonal antibody from mouse

Anticoagulant: /

Immunogen: NSO, V35-N242

Gene name: T-lymphocyte activation antigen CD80 / CD80 molecule

Protein name: T-lymphocyte activation antigen CD80

Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)


|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|162|445|1656|163|425|1791
Standard deviation|7.29|34.71|69.55|7.98|34|100.29
CV(%)|4.5%|7.8%|4.2%|4.9%|8%|5.6%


Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 162 138 164 139 150 12.27 8.1%
Sample 2 445 495 515 457 478 28.23 5.9%
Sample 3 1656 1667 1402 1560 1571 106.21 6.7%

*number of samples for each test n=16.


Catalog number|Description|Quantity
EK0707-CAP|96-well plate precoated with anti- human CD80 antibody|1
EK0707-ST|Lyophilized recombinant human CD80 standard|10ng/tube×2
EK0707-DA|Biotinylated anti- human CD80 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4