Description
Boster’s human Autotaxin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Autotaxin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A36-I863) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Autotaxin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Autotaxin amount of sample captured in plate.
Synonyms: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; Autotaxin; Extracellular lysophospholipase D; LysoPLD; ENPP2; ATX; PDNP2
Species: Human Autotaxin
Assay: 625pg/ml-40000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human total Autotaxin
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO,A36-I863
Gene name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 / ectonucleotide pyrophosphatase/phosphodiesterase 2
Protein name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1266|4575|22840|1326|4349|24036
Standard deviation|87.35|297.37|1256.2|92.82|287.03|1562.34
CV(%)|6.9%|6.5%|5.5%|7%|6.6%|6.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1266 | 1230 | 1334 | 1188 | 1254 | 53.56 | 4.2% |
| Sample 2 | 4575 | 4258 | 4827 | 4594 | 4563 | 202.37 | 4.4% |
| Sample 3 | 22840 | 25398 | 23227 | 24614 | 24019 | 1033.55 | 4.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1655-CAP|96-well plate precoated with anti- human Autotaxin antibody|1
EK1655-ST|Lyophilized recombinant human Autotaxin standard|40ng/tube×2
EK1655-DA|Biotinylated anti- human Autotaxin antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
