Description
Boster’s human ADAMTS13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS13 has been precoated onto 96-well plates. Standards(CHO, Q34-W688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS13 amount of sample captured in plate.
Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 13|A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13|A disintegrin like and metalloprotease with thrombospondin type 1 motif 13|A disintegrin-like and metalloprotease (Reprolysin type) with thrombospondin type 1 motif 13|ADAM metallopeptidase with thrombospondin type 1 motif 13|ADAM TS|ADAM-TS 13|ADAM-TS13|ADAMTS 13|ADAMTS-13|ADAMTS13|ADAMTS13 protein|ATS13_HUMAN|C9orf8|EC 3.4.24.-|TTP|Von Willebrand factor cleaving protease|Von Willebrand factor cleaving protease|von Willebrand factor-cleaving protease|vWF cleaving protease|vWF CP|vWF-cleaving protease|vWF-CP|vWFCP
Species: Human ADAMTS13
Assay: 0.78ng/ml-50ng/ml
Sensitivy: <20pg/ml Specifity: Natural and recombinant human ADAMTS13
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: CHO, Q34-W688
Gene name: A disintegrin and metalloproteinase with thrombospondin motifs 13 / ADAM metallopeptidase with thrombospondin type 1 motif, 13
Protein name: A disintegrin and metalloproteinase with thrombospondin motifs 13
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1395|7236|20816|1322|6617|20301
Standard deviation|104.62|289.44|1165.69|104.43|291.14|1197.75
CV(%)|7.5%|4%|5.6%|7.9%|4.4%|5.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1395 | 1323 | 1392 | 1567 | 1419 | 90.03 | 6.3% |
| Sample 2 | 7236 | 8286 | 7812 | 8064 | 7849 | 391.89 | 4.9% |
| Sample 3 | 20816 | 20291 | 22433 | 22964 | 21626 | 1104.51 | 5.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0927-CAP|96-well plate precoated with anti- human ADAMTS13 antibody|1
EK0927-ST|Lyophilized recombinant human ADAMTS13 standard|50ng/tube×2
EK0927-DA|Biotinylated anti- human ADAMTS13 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
