Description
Boster’s human ADAM8 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM8 has been precoated onto 96-well plates. Standards(NSO, M1-P497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM8 amount of sample captured in plate.
Synonyms: A Disintegrin And Metalloproteinase domain 8|A Disintegrin And Metalloproteinase domain 8|ADAM 8|ADAM 8 precursor|ADAM 8 precursor| ADAM metallopeptidase domain 8|Adam8|ADAM8 protein|ADAM8_HUMAN|CD 156|CD156|CD156a|CD156a antigen|CD156a antigen|Cell surface antigen MS2|Cell surface antigen MS2|Disintegrin and metalloproteinase domain-containing protein 8|Human leukocyte differentiation antigen|Human leukocyte differentiation antigen|Macrophage cysteine rich glycoprotein|Macrophage cysteine rich glycoprotein| MGC134985|MS 2|MS2
Species: Human ADAM8
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human ADAM8
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO, M1-P497
Gene name: Disintegrin and metalloproteinase domain-containing protein 8 / ADAM metallopeptidase domain 8
Protein name: Disintegrin and metalloproteinase domain-containing protein 8
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|143|493|1805|150|511|1879
Standard deviation|10.72|27.6|97.47|11.25|33.72|127.77
CV(%)|7.5%|5.6%|5.4%|7.5%|6.6%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 143 | 150 | 153 | 145 | 147 | 3.96 | 2.6% |
| Sample 2 | 493 | 533 | 465 | 527 | 504 | 27.43 | 5.4% |
| Sample 3 | 1805 | 1971 | 1907 | 1936 | 1904 | 61.88 | 3.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0650-CAP|96-well plate precoated with anti- human ADAM8antibody|1
EK0650-ST|Lyophilized recombinant human ADAM8 standard|10ng/tube×2
EK0650-DA|Biotinylated anti- human ADAM8 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
