The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis. HIF-1α has emerged as an important drug target in breast and prostate cancer

HIF-1α Transcription Factor Assay Kit – 96 wells

Brand:
Cayman
Storage:
-80

The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis.{11044,12428} HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia.{10485,10724,11835} Cayman’s HIF-1α Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysate. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) consensus sequence containing the HIF-1α response element is immobilized to the wells of a 96-well plate. HIF-1α contained in a nuclear extract or whole cell lysates, binds specifically to the HIF-1α response element. The HIF transcription factor complex is detected by addition of a specific primary antibody directed against HIF-1α. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.  

 

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SKU: 10006910 - 96 wells Category:

Description

The HIF (hypoxia-inducible factor) transcription factor is a member of the basic-helix-loop-helix (bHLH) family of transcription factors and plays an important role in maintaining cellular oxygen homeostasis. HIF-1α has emerged as an important drug target in breast and prostate cancer, cardiovascular disease, and ischemia. Cayman’s HIF-1αTranscription Factor Assay is a sensitive ELISA-based method for detecting HIF-1α DNA binding activity in nuclear extracts and whole cell lysates.

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Synonyms:  Hypoxia-Inducible Factor-1α

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Origin: Animal/Bovine|Animal/Rabbit|Animal/Goat

Product Type|Assay Kits|ELISA||Research Area|Cancer|Transcription Factors|HIF-1α||Research Area|Cancer|Tumor Microenvironment||Research Area|Cardiovascular System|Cardiovascular Diseases||Research Area|Epigenetics, Transcription, & Translation|Transcription Factors

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