Dog Canine TGF Beta 1 PicoKine™ ELISA Kit

Brand:
Boster Bio
Storage:

Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Dog Caninea TGF beta 1. 96wells/kit, with removable strips.   

 

Available on backorder

SKU: EK0513-CN Category:

Description

Boster’s Canine TGF beta 1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF beta 1 has been precoated onto 96-well plates. Standards(CHO, A279-S390) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF beta 1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Canine TGF beta 1 amount of sample captured in plate.


Synonyms: Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;TGFB1;TGFB;

Species: Canine TGFB1

Assay: 15.6pg/ml-1000pg/ml

Sensitivy: <1pg/ml Specifity:

Cross Reactivity: There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%. Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat

Anticoagulant: EDTA

Immunogen: Expression system for standard: CHO; Immunogen sequence: A279-S390

Gene name: Transforming growth factor beta-1 / Transforming growth factor beta-1

Protein name: Transforming growth factor beta-1

Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)


Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 24 24 24
Mean(pg/ml) 135 264 521 152 270 543
Standard deviation 3.64 13.73 18.8 7.6 17.3 32.04
CV(%) 4.4 5.2 3.6 5 6.4 5.9

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%)
Sample 1 30 29 32 28 29 1.47 5%
Sample 2 156 166 150 158 157 5.72 3.6%
Sample 3 497 448 471 476 473 17.42 3.6%

*number of samples for each test n=16.


10ng/tube