Annexin A1 Polyclonal Antibody – 100 µg

Annexins are a superfamily of 13 proteins sharing a high degree of homology. They have in common a core C-terminal domain containing calcium and phospholipid binding motifs, allowing most of them to bind to phospholipid membranes in a calcium-dependent manner. The N-terminal domains vary between family members and provide unique function.{30992} Annexin A1 is an endogenous mediator of inflammation, promoting resolution in a number of ways. Normally expressed in intracellular compartments, it is drawn to the cell membrane and both induced and externalized by glucocorticoid response pathways.{30993,30994} The glucocorticoid-induced production and release of annexin A1 is the primary means by which glucocorticoids function as anti-inflammatory agents. Annexin A1 inhibits the synthesis of pro-inflammatory eicosanoids by suppressing the function of sPLA2. This, in turn, limits the recruitment of neutrophils into inflammatory sites and down-regulates the production of pro-inflammatory mediators by those neutrophils that enter inflammatory sites.{30995} Meanwhile, proteolytic fragments generated in response to increased expression are implicated in producing a marker for phagocytosis.{30996} Annexin A1 also functions in the resolution of inflammation by inducing neutrophil apoptosis, and promoting neutrophil clearance (efferocytosis) by macrophages. The pro-resolving functions of annexin A1 are mediated via binding to FPR2/ALX, a receptor it shares with the specific pro-resolving mediators lipoxin A4 (LXA4; Item No. 90410) and resolvin D1 (RvD1; Item No. 10012554).{30995} The molecule’s regulatory role has led to investigation of the downstream effects of annexin A1, including cancer, adaptive immunity, and wound repair.{30997,30998,30999} The predicted size of annexin A1 is 38.7 kDa and Cayman’s Annexin A1 Polyclonal Antibody detects a size 39 kDa band via Western blot and Immunoprecipitation.