Cayman’s α-KG Fluorometric Detection Assay provides a fluorescence-based method for quantifying α-KG in biological samples such as serum

α-Ketoglutarate Fluorometric Detection Assay Kit – 96 wells

Brand:
Cayman
Storage:
-20

Cayman’s α-Ketoglutarate (α-KG) Fluorometric Detection Assay provides a fluorescence-based method for quantifying α-KG in biological samples such as serum, plasma, urine, and tissue. It can also be utilized to determine intracellular and extracellular α-KG concentrations in cell culture samples. In this assay, alanine transaminase (ALT) deaminates α-KG to form pyruvate.{33073,33074} Pyruvate is then oxidized by pyruvate oxidase to yield acetyl phosphate, hydrogen peroxide (H2O2), and carbon dioxide.{33075} As H2O2 is produced, the cycling mechanism of horseradish peroxidase results in the concurrent reduction of H2O2 to H2O and oxidation of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) to produce the highly fluorescent compound resorufin.{33076} Resorufin fluorescence is analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm.  

 

Available on backorder

Add to quotes
SKU: 701350 - 96 wells Category:

Description

Cayman’s α-KG Fluorometric Detection Assay provides a fluorescence-based method for quantifying α-KG in biological samples such as serum, plasma, urine, and tissue. It can also be utilized to determine intracellular and extracellular α-ketoglutarate concentrations in cell culture samples.

Formulation:

Formal name:

Synonyms: 

Host:

Imunogen:

Applications: 

Clone:

Purity:

Origin: Animal/Bovine

Product Type|Assay Kits|Detection Kits||Product Type|Assay Kits|Fluorometric Assays||Research Area|Cell Biology|Mitochondrial Biology||Research Area|Endocrinology & Metabolism

Related products