Description
Boster’s mouse OPG ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for OPG has been precoated onto 96-well plates. Standards(NSO, E22-L401) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OPG is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse OPG amount of sample captured in plate.
Synonyms: Tumor necrosis factor receptor superfamily member 11B;Osteoclastogenesis inhibitory factor;Osteoprotegerin;Tnfrsf11b;Ocif, Opg;
Species: Mouse OPG
Assay: 93.7pg/ml-6000pg/ml
Sensitivy: <5pg/ml Specifity: Natural and recombinant mouse OPG
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from rat|polyclonal antibody from goat
Anticoagulant: EDTA
Immunogen: Expression system for standard: NSO; Immunogen sequence: E22-L401
Gene name: Tumor necrosis factor receptor superfamily member 11B / Tumor necrosis factor receptor superfamily member 11B
Protein name: Tumor necrosis factor receptor superfamily member 11B
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 133 | 800 | 3535 | 139 | 767 | 3643 |
| Standard deviation | 5.85 | 54.4 | 187.35 | 7.3%9 | 55.3%9 | 225.86 |
| CV(%) | 4.4% | 6.8% | 5.3% | 4.6% | 7.3% | 6.2% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 133 | 157 | 157 | 146 | 148 | 9.88 | 6.6% |
| Sample 2 | 800 | 766 | 743 | 710 | 754 | 32.84 | 4.3% |
| Sample 3 | 3535 | 3949 | 3581 | 3584 | 3662 | 166.69 | 4.5% |
*number of samples for each test n=16.
10ng/tube
