Description
Boster’s human FASL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FASL has been precoated onto 96-well plates. Standards(CHO, P134-L281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FASL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FASL amount of sample captured in plate.
Synonyms: Tumor necrosis factor ligand superfamily member 6;Apoptosis antigen ligand;APTL;CD95 ligand;CD95-L;Fas antigen ligand;Fas ligand;FasL;CD178;Tumor necrosis factor ligand superfamily member 6, membrane form;Tumor necrosis factor ligand superfamily member 6, soluble form;Receptor-binding FasL ectodomain;Soluble Fas ligand;sFasL;ADAM10-processed FasL form;APL;FasL intracellular domain;FasL ICD;SPPL2A-processed FasL form;SPA;FASLG;APT1LG1, CD95L, FASL, TNFSF6;
Species: Human FASL
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant human FASL
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: Expression system for standard: CHO; Immunogen sequence: P134-L281
Gene name: Tumor necrosis factor ligand superfamily member 6 / Tumor necrosis factor ligand superfamily member 6
Protein name: Tumor necrosis factor ligand superfamily member 6
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 36 | 186 | 437 | 34 | 189 | 419 |
| Standard deviation | 2.19 | 9.67 | 28.84 | 2.1 | 12.47 | 33.1 |
| CV(%) | 6.1% | 5.2% | 6.6% | 6.2% | 6.6% | 7.9% |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 41 | 41 | 34 | 38 | 3.08 | 8.1% |
| Sample 2 | 186 | 197 | 192 | 198 | 193 | 4.76 | 2.4% |
| Sample 3 | 437 | 495 | 446 | 507 | 471 | 30.21 | 6.4% |
*number of samples for each test n=16.
10ng/tube
