Description
Boster’s Bovine Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Bovine Activin A amount of sample captured in plate.
Synonyms: Inhibin, beta A (Activin A, activin AB alpha polypeptide) ;Inhibin, beta A (Activin A, activin AB alpha polypeptide), isoform CRA_a ;cDNA FLJ75379, highly similar to Homo sapiens inhibin, beta A (activin A, activin AB alpha polypeptide), mRNA ;INHBA ;hCG_17267 , tcag7.474 ;
Species: Bovine INHBA
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <12pg/ml Specifity:
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: monoclonal antibody from mouse|monoclonal antibody from mouse
Anticoagulant: heparin or EDTA
Immunogen: Expression system for standard: CHO; Immunogen sequence: G311-S426
Gene name: Inhibin beta A chain / inhibin, beta A
Protein name: Inhibin beta A chain
Storage: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3_x000D_ |
| n | 16 | 16 | 16 | 24 | 24 | 24_x000D_ |
| Mean(pg/ml) | 90 | 281 | 467 | 95 | 293 | 489_x000D_ |
| Standard deviation | 4.7 | 15.7 | 27.6 | 7.8 | 18.5 | 24.9_x000D_ |
| CV(%) | 5.2 | 5.6 | 5.9 | 8.2 | 6.3 | 5.1 |
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 40 | 35 | 33 | 35 | 2.69 | 7.6% |
| Sample 2 | 199 | 204 | 220 | 225 | 212 | 10.79 | 5% |
| Sample 3 | 467 | 440 | 464 | 464 | 458 | 10.89 | 2.3% |
*number of samples for each test n=16.
10ng/tube
