Description
Boster’s human APLP1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for APLP1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S39-E580) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for APLP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human APLP1 amount of sample captured in plate.
Synonyms: Amyloid-like protein 1; APLP; APLP-1; C30; APLP1
Species: Human APLP1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant human APLP1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated monoclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, S39-E580
Gene name: Amyloid-like protein 1 / amyloid beta precursor like protein 1
Protein name: Amyloid-like protein 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|252|1375|5201|272|1440|5518
Standard deviation|15.37|90.75|358.86|17.68|102.24|452.47
CV(%)|6.1%|6.6%|6.9%|6.5%|7.1%|8.2%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 252 | 209 | 251 | 225 | 234 | 18.15 | 7.7% |
| Sample 2 | 1375 | 1410 | 1341 | 1498 | 1406 | 58.45 | 4.1% |
| Sample 3 | 5201 | 4542 | 5134 | 5110 | 4996 | 264.65 | 5.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK2103-CAP|96-well plate precoated with anti- human APLP1 antibody|1
EK2103-ST|Lyophilized recombinant human APLP1 standard|10ng/tube×2
EK2103-DA|Biotinylated anti- human APLP1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
