Description
Boster’s mouse RTN4R ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for RTN4R has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: C27-S447) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for RTN4R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse RTN4R amount of sample captured in plate.
Synonyms: Reticulon-4 receptor; Nogo receptor; NgR; Nogo-66 receptor; Nogo66 receptor-1; NgR1; Rtn4r; Ngr1; Nogor
Species: Mouse RTN4R
Assay: 156pg/ml-10000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant mouse RTN4R
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO, C27-S447
Gene name: Reticulon-4 receptor / reticulon 4 receptor
Protein name: Reticulon-4 receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|301|1170|4798|307|1195|4812
Standard deviation|15.65|62.01|259.09|18.73|70.51|327.22
CV(%)|5.2%|5.3%|5.4%|6.1%|5.9%|6.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 301 | 299 | 261 | 285 | 286 | 15.96 | 5.5% |
| Sample 2 | 1170 | 1314 | 1144 | 1295 | 1230 | 74.62 | 6.0% |
| Sample 3 | 4798 | 4625 | 4283 | 4662 | 4592 | 189.67 | 4.1% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK2066-CAP|96-well plate precoated with anti- mouse RTN4R antibody|1
EK2066-ST|Lyophilized recombinant mouse RTN4R standard|10ng/tube×2
EK2066-DA|Biotinylated anti- mouse RTN4R antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
