Description
Boster’s human Brevican ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Brevican has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: D23-P911) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Brevican is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Brevican amount of sample captured in plate.
Synonyms: Brevican core protein; Brain-enriched hyaluronan-binding protein; BEHAB; Chondroitin sulfate proteoglycan 7; BCAN; BEHAB; CSPG7; UNQ2525;PRO6018
Species: Human Brevican
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human Brevican
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, D23-P911
Gene name: Brevican core protein / brevican
Protein name: Brevican core protein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|53|208|824|57|214|958
Standard deviation|2.23|8.94|33.78|3.76|14.55|72.81
CV(%)|4.2%|4.3%|4.1%|6.6%|6.8%|7.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 53 | 50 | 49 | 55 | 51 | 2.38 | 4.6% |
| Sample 2 | 208 | 199 | 203 | 231 | 210 | 12.39 | 5.9% |
| Sample 3 | 824 | 819 | 818 | 777 | 809 | 18.90 | 2.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK2019-CAP|96-well plate precoated with anti- human Brevican antibody|1
EK2019-ST|Lyophilized recombinant human Brevican standard|10ng/tube×2
EK2019-DA|Biotinylated anti- human Brevican antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
