Description
Boster’s rat SEZ6L ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SEZ6L has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: L29-A958) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RD is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat SEZ6L amount of sample captured in plate.
Synonyms: Seizure-related 6 homolog-like; Sez6l
Species: Rat SEZ6L
Assay: 156pg/ml-10000pg/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant rat SEZ6L
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, L29-A958
Gene name: Seizure-related 6 homolog-like / seizure related 6 homolog like
Protein name: Seizure-related 6 homolog-like
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|176|807|4126|260|941|4859
Standard deviation|8.62|34.70|169.17|15.60|54.58|335.27
CV(%)|4.9%|4.3%|4.1%|6.0%|5.8%|6.9%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 176 | 199 | 178 | 190 | 185 | 9.33 | 5.0% |
| Sample 2 | 807 | 825 | 758 | 743 | 783 | 33.78 | 4.3% |
| Sample 3 | 4126 | 3875 | 3712 | 3928 | 3910 | 147.82 | 3.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1832-CAP|96-well plate precoated with anti- rat SEZ6L antibody|1
EK1832-ST|Lyophilized recombinant rat SEZ6L standard|10ng/tube×2
EK1832-DA|Biotinylated anti- rat SEZ6L antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
