Description
Boster’s rat IL6ST ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL6ST has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q23-E618) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL6ST is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Synonyms: Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; Il6st
Species: Rat IL6ST
Assay: 0.78ng/ml-50ng/ml
Sensitivy: <0.05ng/ml Specifity: Natural and recombinant rat IL6ST
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: NSO, Q23-E618
Gene name: Interleukin-6 receptor subunit beta / interleukin 6 signal transducer
Protein name: Interleukin-6 receptor subunit beta
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1254|4579|20188|1322|5546|21456
Standard deviation|66.46|290.3|847.9|99.15|321.7|1652.1
CV(%)|5.3%|6.1%|4.2%|7.5%|5.8%|7.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1254 | 1275 | 1273 | 1502 | 1326 | 101.94 | 7.6% |
| Sample 2 | 4597 | 4887 | 4558 | 4251 | 4573 | 225.32 | 4.9% |
| Sample 3 | 20188 | 20369 | 19931 | 19988 | 20119 | 173.03 | 0.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1742-CAP|96-well plate precoated with anti- rat IL6ST antibody|1
EK1742-ST|Lyophilized recombinant rat IL6ST standard|50ng/tube×2
EK1742-DA|Biotinylated anti- rat IL6ST antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
