Description
Boster’s mouse AAT ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for AAT has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: E25-K413） and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AAT is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse AAT amount of sample captured in plate.
Synonyms: Fibroblast growth factor-binding protein 1; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; 17 kDa heparin-binding growth factor-binding protein; 17 kDa HBGF-binding protein; HBp17; FGFBP1; FGFBP; HBP17
Species: Mouse AAT
Assay: 1.56ng/ml-100ng/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse AAT
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,E25-K413
Gene name: Alpha-1-antitrypsin 1-2 / signal transducer and activator of transcription 3
Protein name: Alpha-1-antitrypsin 1-2
Storage: Store at 4˚C for 6 months, at -20˚C for 15 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|2876|13987|37231|2911|14122|4269
Standard deviation|158.18|713.3|1600.9|177.57|932.05|320.18
CV(%)|5.5%|5.1%|4.3%|6.1%|6.6%|7.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 2876 | 2791 | 2438 | 2616 | 2680 | 168.37 | 6.2% |
| Sample . 2 |
13987 | 13721 | 13461 | 14393 | 13890 | 344.60 | 2.4%% |
| Sample 3 | 37231 | 37794 | 38247 | 41965 | 38809 | 1857.18 | 4.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1711-CAP|96-well plate precoated with anti- mouse AAT antibody|1
EK1711-ST|Lyophilized recombinant mouse AAT standard|100ng/tubex2
EK1711-DA|Biotinylated anti- mouse AAT antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
