Description
Boster’s mouse PGLYRP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PGLYRP1 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: F19-E182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PGLYRP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PGLYRP1 amount of sample captured in plate.
Synonyms: Peptidoglycan recognition protein 1|Cytokine tag7|Peptidoglycan recognition protein short|PGRP-S|Pglyrp1|Pglyrp|Pgrp|Pgrps|Tag7
Species: Mouse Pglyrp1
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse PGLYRP1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, F19-E182
Gene name: Peptidoglycan recognition protein 1 / peptidoglycan recognition protein 1
Protein name: Peptidoglycan recognition protein 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|34|223|498|37|211|460
Standard deviation|1.87|16.05|27.39|2.03|16.24|26.68
CV(%)|5.5%|7.2%|5.5%|5.5%|7.7%|5.8%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 34 | 31 | 32 | 32 | 32 | 1.08 | 3.3% |
| Sample 2 | 223 | 229 | 202 | 199 | 213 | 12.96 | 6% |
| Sample 3 | 498 | 462 | 442 | 494 | 474 | 23.15 | 4.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity_x000D_
EK1618-CAP|96-well plate precoated with anti- mouse PGLYRP1 antibody|1_x000D_
EK1618-ST|Lyophilized recombinant mouse PGLYRP1 standard|10ng/tube×2_x000D_
EK1618-DA|Biotinylated anti- mouse PGLYRP1 antibody|130μl(dilution 1:100)_x000D_
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)_x000D_
AR1106-1|Sample diluent buffer|30ml_x000D_
AR1106-2|Antibody diluent buffer|12ml_x000D_
AR1106-3|ABC diluent buffer|12ml_x000D_
AR1104|TMB color developing agent|10ml_x000D_
AR1105|TMB stop solution|10ml_x000D_
PLA-SEA|Adhesive cover|4
