Description
Boster’s rat TRKB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TRKB has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: C32-H429） and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TRKB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TRKB amount of sample captured in plate.
Synonyms: BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; Trk-B; Ntrk2; Trkb
Species: Rat TRKB
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant rat TRKB
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,C32-H429
Gene name: BDNF/NT-3 growth factors receptor / neurotrophic receptor tyrosine kinase 2
Protein name: BDNF/NT-3 growth factors receptor
Storage: Store at 4˚C for 6 months, at -20˚C for 15 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|60|296|737|61|301|845
Standard deviation|3.18|16.28|47.9|2.81|20.8|65.07
CV(%)|5.3%|5.5%|6.5%|4.6%|6.9%|7.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 60 | 59 | 67 | 59 | 61 | 3.34 | 5.4% |
| Sample . 2 |
296 | 309 | 308 | 268 | 295 | 16.54 | 5.6%% |
| Sample 3 | 737 | 842 | 738 | 741 | 764 | 44.76 | 5.8% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1596-CAP|96-well plate precoated with anti- rat TRKB antibody|1
EK1596-ST|Lyophilized recombinant rat TRKB standard|10ng/tubex2
EK1596-DA|Biotinylated anti- rat TRKB antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
