Description
Boster’s human CHL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CHL1 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: I25-Q1096) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CHL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CHL1 amount of sample captured in plate.
Synonyms: CALL | Cell adhesion molecule with homology to L1CAM (close homolog of L1) | Close homolog of L1 | FLJ44930 | L1 cell adhesion molecule 2 | L1CAM2 | MGC132578 | Neural cell adhesion molecule | O00533
Species: Human CHL1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CHL1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, I25-Q1096
Gene name: Neural cell adhesion molecule L1-like protein / cell adhesion molecule L1 like
Protein name: Neural cell adhesion molecule L1-like protein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|291|1965|5542|267|2117|5729
Standard deviation|22.11|141.48|343.6|21.09|192.64|435.4
CV(%)|7.6%|7.2%|6.2%|7.9%|9.1%|7.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 291 | 285 | 268 | 269 | 278 | 9.98 | 3.5% |
| Sample 2 | 1965 | 1964 | 2077 | 2085 | 2022 | 58.31 | 2.8% |
| Sample 3 | 5542 | 5306 | 5133 | 4837 | 5204 | 257.08 | 4.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1576-CAP|96-well plate precoated with anti- human CHL1 antibody|1
EK1576-ST|Lyophilized recombinant human CHL1 standard|10ng/tube×2
EK1576-DA|Biotinylated anti- human CHL1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
