Description
Boster’s human CD47 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD47 has been precoated onto 96-well plates. Standards (NSO, Q19 – P139) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD47 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD47 amount of sample captured in plate.
Synonyms: Antigen identified by monoclonal antibody 1D8|Antigenic surface determinant protein OA3|CD 47|CD47|CD47 antigen (Rh-related antigen, integrin-associated signal transducer)|CD47 antigen|CD47 glycoprotein|CD47 molecule|CD47_HUMAN|IAP|Integrin Associated Protein|
Integrin associated signal transducer|Integrin-associated protein|Leukocyte surface antigen CD47|MER6|OA 3|OA3|OTTHUMP00000041152|
OTTHUMP00000041153|Protein MER6|Rh related antigen
Species: human CD47
Assay: 125pg/ml-8000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD47
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: /
Immunogen: NSO,Q19-P139
Gene name: Leukocyte surface antigen CD47 / CD47 molecule
Protein name: Leukocyte surface antigen CD47
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|193|888|3988|208|813|3951
Standard deviation|7.72|54.16|183.44|11.02|60.97|256.81
CV(%)|4%|6.1%|4.6%|5.3%|7.5%|6.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 193 | 162 | 183 | 189 | 181 | 11.94 | 6.5% |
| Sample 2 | 888 | 960 | 1024 | 1007 | 969 | 52.69 | 5.4% |
| Sample 3 | 3988 | 4066 | 3941 | 3907 | 3975 | 59.64 | 1.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1421-CAP|96-well plate precoated with anti- human CD47 antibody|1
EK1421-ST|Lyophilized recombinant human CD47 standard|10ng/tube×2
EK1421-DA|Biotinylated anti- human CD47 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
