Description
Boster’s human CD44 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD44 has been precoated onto 96-well plates. Standards(NSO, Gln21 – Pro220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD44 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD44 amount of sample captured in plate.
Synonyms: LHR|BA-1|CD 44|CD44|CD44 antigen|CD44 molecule(Indian blood group)|CD44 molecule|CD44_HUMAN|CDw44|Cell surface glycoprotein CD44| chondroitin sulfate proteoglycan 8|CSPG8|ECMR-III|Epican|Extracellular matrix receptor III|GP90 lymphocyte homing/adhesion receptor| HCELL|hematopoietic cell E- and L-selectin ligand|Heparan sulfate proteoglycan|Hermes antigen |homing function and Indian blood group system|HSA|HUTCH-I|HUTCH1|Hyaluronate receptor|IN|LHR|MC56|MDU2|MDU3|MGC10468|MIC4|MUTCH1|PGP-1|PGP-I|PGP1|Phagocytic glycoprotein 1| Phagocytic glycoprotein I
Species: human CD44
Assay: 0.78ng/ml-50ng/ml
Sensitivy: <20pg/ml Specifity: Natural and recombinant human CD44
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO,Gln21-Pro220
Gene name: CD44 antigen / CD44 molecule(Indian blood group)
Protein name: CD44 antigen
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1896|4142|25002|2011|3769|27132
Standard deviation|102.38|219.52|1525.12|138.75|244.98|1817.84
CV(%)|5.4%|5.3%|6.1%|6.9%|6.5%|6.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1896 | 1710 | 1653 | 1784 | 1760 | 90.85 | 5.1% |
| Sample 2 | 4142 | 4287 | 4199 | 4238 | 4216 | 53.12 | 1.2% |
| Sample 3 | 25002 | 23581 | 25055 | 23252 | 24222 | 814.56 | 3.3% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1418-CAP|96-well plate precoated with anti- human CD44 antibody|1
EK1418-ST|Lyophilized recombinant human CD44 standard|50ng/tube×2
EK1418-DA|Biotinylated anti- human CD44 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
