Description
Boster’s human LOXL2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for LOXL2 has been precoated onto 96-well plates. Standards (NSO,Q26-Q774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for LOXL2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human LOXL2 amount of sample captured in plate.
Synonyms: LOR 2|LOR2|LOX L2|LOXL 2|LOXL2|LOXL2_HUMAN|Lysyl oxidase homolog 2|Lysyl oxidase like 2|Lysyl oxidase like protein 2|Lysyl oxidase related 2|Lysyl oxidase related protein 2|Lysyl oxidase related protein WS9 14|Lysyl oxidase-like protein 2|Lysyl oxidase-related protein 2|Lysyl oxidase-related protein WS9-14|WS9 14
Species: human LOXL2
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human LOXL2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, Q26-Q774
Gene name: Lysyl oxidase homolog 2 / lysyl oxidase-like 2
Protein name: Lysyl oxidase homolog 2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|252|1465|3854|263|1490|4228
Standard deviation|11.84|114.27|254.36|13.93|123.67|317.1
CV(%)|4.7%|7.8%|6.6%|5.3%|8.3%|7.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 252 | 291 | 276 | 299 | 279 | 17.89 | 6.4% |
| Sample 2 | 1465 | 1420 | 1599 | 1469 | 1488 | 66.77 | 4.4% |
| Sample 3 | 3854 | 3928 | 3993 | 3849 | 3906 | 59.17 | 1.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1391-CAP|96-well plate precoated with anti- human LOXL2 antibody|1
EK1391-ST|Lyophilized recombinant human LOXL2 standard|10ng/tube×2
EK1391-DA|Biotinylated anti- human LOXL2 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
