Description
Boster’s human CEACAM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CEACAM1 has been precoated onto 96-well plates. Standards(NSO, Q35-G428) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CEACAM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CEACAM1 amount of sample captured in plate.
Synonyms: Antigen CD66|BGP|BGP 1|BGPI|Biliary glycoprotein|Biliary glycoprotein 1|Biliary glycoprotein adhesion molecule|Carcinoembryonic antigen related cell adhesion molecule 1|CD66a|CEA related cell adhesion molecule|CEACAM 1|CEACAM1
Species: human CEACAM1
Assay: 312pg/ml-20000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CEACAM1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, Q35-G428
Gene name: Carcinoembryonic antigen-related cell adhesion molecule 1 / carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein)
Protein name: Carcinoembryonic antigen-related cell adhesion molecule 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|463|2835|9439|432|3010|9810
Standard deviation|32.41|150.25|453.07|35.42|207.69|529.74
CV(%)|7%|5.3%|4.8%|8.2%|6.9%|5.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 463 | 407 | 434 | 425 | 432 | 20.24 | 4.6% |
| Sample 2 | 2835 | 2793 | 2564 | 2668 | 2715 | 106.64 | 3.9% |
| Sample 3 | 9439 | 8061 | 8668 | 8372 | 8635 | 511.4 | 5.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1361-CAP|96-well plate precoated with anti- human CEACAM1 antibody|1
EK1361-ST|Lyophilized recombinant human CEACAM1 standard|20ng/tube×2
EK1361-DA|Biotinylated anti- human CEACAM1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
