Description
Boster’s mouse CD22 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD22 has been precoated onto 96-well plates. Standards(NSO, S22-R702) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD22 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD22 amount of sample captured in plate.
Synonyms: B cell receptor CD22 precursor|B lymphocyte cell adhesion molecule|B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL CAM|BL-CAM|BLCAM|CD 22|CD22|CD22 antigen|CD22 molecule|CD22 protein|CD22_HUMAN|Lectin 2|Leu14|Lyb8|MGC130020|sialic acid binding Ig like lectin 2|Sialic acid binding immunoglobulin like lectin 2|Sialic acid-binding Ig-like lectin 2|SIGLEC 2|Siglec-2|SIGLEC2|T cell surface antigen Leu 14|T-cell surface antigen Leu-14
Species: mouse CD22
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant mouse CD22
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, S22-R702
Gene name: B-cell receptor CD22 / CD22 molecule
Protein name: B-cell receptor CD22
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|240|2070|4058|246|1914|3971
Standard deviation|16.56|124.2|182.61|18.94|132.06|218.4
CV(%)|6.9%|6%|4.5%|7.7%|6.9%|5.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 240 | 242 | 251 | 262 | 248 | 8.69 | 3.5% |
| Sample 2 | 2070 | 1778 | 1779 | 2120 | 1936 | 159.23 | 8.2% |
| Sample 3 | 4058 | 4096 | 4754 | 4460 | 4342 | 284.97 | 6.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1303-CAP|96-well plate precoated with anti- mouse CD22 antibody|1
EK1303-ST|Lyophilized recombinant mouse CD22 standard|10ng/tube×2
EK1303-DA|Biotinylated anti- mouse CD22 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
