Description
Boster’s human Ficolin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-2 has been precoated onto 96-well plates. Standards(NSO, L26-A313) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-2 amount of sample captured in plate.
Synonyms: 37 kDa elastin-binding protein|Collagen/fibrinogen domain containing protein 2|Collagen/fibrinogen domain-containing protein 2|EBP 37|EBP-37|EBP37|FCN 2|Fcn2|FCN2_HUMAN|FCNL|Ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)|Ficolin B|Ficolin beta|Ficolin-2|Ficolin-B|Ficolin-beta|Ficolin2|Hucolin|L ficolin|L-ficolin|OTTHUMP00000022518|P35|RP11 263F14.2|Serum lectin p35
Species: human Ficolin-2
Assay: 0.78ng/ml-50ng/ml
Sensitivy: <20pg/ml Specifity: Natural and recombinant human Ficolin-2
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin
Immunogen: NSO, L26-A313
Gene name: Ficolin-2 / ficolin (collagen/fibrinogen domain containing lectin) 2
Protein name: Ficolin-2
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|977|6812|22201|979|6528|20934
Standard deviation|44.94|497.27|1354.26|52.86|509.18|1611.91
CV(%)|4.6%|7.3%|6.1%|5.4%|7.8%|7.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 977 | 1058 | 1110 | 1008 | 1038 | 50.5 | 4.8% |
| Sample 2 | 6812 | 6337 | 6515 | 7162 | 6706 | 312.97 | 4.6% |
| Sample 3 | 22201 | 22603 | 22446 | 21704 | 22238 | 340.22 | 1.5% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK1258-CAP|96-well plate precoated with anti- human Ficolin-2 antibody|1
EK1258-ST|Lyophilized recombinant human Ficolin-2 standard|50ng/tube×2
EK1258-DA|Biotinylated anti- human Ficolin-2 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
