Description
Boster’s human TNFRSF16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TNFRSF16 has been precoated onto 96-well plates. Standards (sf21, K29 – N250) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TNFRSF16 amount of sample captured in plate.
Synonyms: CD271|CD271 antigen|Gp80 LNGFR|Gp80 LNGFR|Gp80-LNGFR|Low affinity nerve growth factor receptor|Low affinity nerve growth factor receptor|Low affinity neurotrophin receptor p75NTR|Low affinity neurotrophin receptor p75NTR|Low-affinity nerve growth factor receptor|Nerve growth factor receptor|Nerve growth factor receptor TNFR superfamily member 16|NGF receptor|NGF receptor|NGFR|p75 ICD|
p75 ICD|p75 Neurotrophin receptor|p75 NTR|p75(NTR)|p75NTR|TNFR Superfamily Member 16|TNFRSF16|TNR16_HUMAN|Tumor necrosis factor receptor superfamily member 16
Species: Human TNFRSF16
Assay: 62.5pg/ml-4000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human TNFRSF16
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: sf21, K29-N250
Gene name: Tumor necrosis factor receptor superfamily member 16 / nerve growth factor receptor
Protein name: Tumor necrosis factor receptor superfamily member 16
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|194|811|1003|200|784|959
Standard deviation|11.44|46.22|52.15|12.6|46.25|53.7
CV(%)|5.9%|5.7%|5.2%|6.3%|5.9%|5.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 194 | 175 | 185 | 176 | 182 | 7.69 | 4.2% |
| Sample 2 | 811 | 686 | 734 | 797 | 757 | 50.21 | 6.6% |
| Sample 3 | 1003 | 1138 | 1104 | 1038 | 1070 | 53.12 | 4.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0972-CAP|96-well plate precoated with anti- human TNFRSF16 antibody|1
EK0972-ST|Lyophilized recombinant human TNFRSF16 standard|10ng/tube×2
EK0972-DA|Biotinylated anti- human TNFRSF16 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
