Description
Boster’s human ANGPTL4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ANGPTL4 has been precoated onto 96-well plates. Standards(NSO, L165-S406) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ANGPTL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ANGPTL4 amount of sample captured in plate.
Synonyms: 425O18 1|_x000D_Angiopoietin like 4|_x000D_Angiopoietin related protein 4|_x000D__x000D__x000D_ANGPT L2 |_x000D_ANGPT L4|_x000D_ANGPTL2|_x000D_ANGPTL4 |_x000D_Fasting induced adipose factor |_x000D_FIAF|_x000D_Fibrinogen/angiopoietin related protein|_x000D_Hepatic fibrinogen/angiopoietin related protein |_x000D_HFARP|_x000D_Major histocompatibility complex region NG27|_x000D_NL2|_x000D_Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein |_x000D_PGAR|_x000D_pp1158 |_x000D_PPARG angiopoietin related protein |_x000D_PSEC0166 |_x000D_Secreted protein Bk89|_x000D_Weakly similar to angiopoietin 1
Species: Human ANGPTL4
Assay: 625pg/ml-40000pg/ml
Sensitivy: <20pg/ml Specifity: Natural and recombinant human ANGPTL4
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, L165-S406
Gene name: Angiopoietin-related protein 4 / angiopoietin-like 4
Protein name: Angiopoietin-related protein 4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1114|4583|20920|1120|4790|21489
Standard deviation|73.52|256.64|941.4|79.52|320.93|988.49
CV(%)|6.6%|5.6%|4.5%|7.1%|6.7%|4.6%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1114 | 1122 | 1295 | 1157 | 1172 | 72.83 | 6.2% |
| Sample 2 | 4583 | 4850 | 5176 | 4540 | 4787 | 253.92 | 5.3% |
| Sample 3 | 20920 | 21440 | 20536 | 18895 | 20447 | 952.15 | 4.6% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0960-CAP|96-well plate precoated with anti- human ANGPTL4 antibody|1
EK0960-ST|Lyophilized recombinant human ANGPTL4 standard|40ng/tube×2
EK0960-DA|Biotinylated anti- human ANGPTL4 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
