Description
Boster’s human CD244 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD244 has been precoated onto 96-well plates. Standards(NSO, C22-R221) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD244 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD244 amount of sample captured in plate.
Synonyms: 2B4|C9.1|CD244|CD244 antigen|CD244 molecule natural killer cell receptor 2B4|CD244 natural killer cell receptor 2B4|CD244_HUMAN|F730046O15Rik|h2B4|Ly90|Natural killer cell activation-inducing ligand|Natural killer cell receptor 2B4|NK cell activation inducing ligand|NK cell activation inducing ligand NAIL|NK cell activation-inducing ligand|NK cell type I receptor protein 2B4|NKR2B4|Nmrk|Non-MHC restricted killing associated|OTTHUMP00000027884|p38|signaling lymphocytic activation molecule 4|SLAM family, member 4|SLAMF4
Species: Human CD244
Assay: 15.6pg/ml-1000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human CD244
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, C22-R221
Gene name: Natural killer cell receptor 2B4 / CD244 molecule, natural killer cell receptor 2B4
Protein name: Natural killer cell receptor 2B4
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|28|81|453|30|112|469
Standard deviation|1.34|4.62|20.84|1.53|6.61|30.02
CV(%)|4.8%|5.7%|4.6%|5.1%|5.9%|6.4%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 28 | 27 | 30 | 28 | 28 | 1.08 | 3.8% |
| Sample 2 | 81 | 79 | 81 | 73 | 78 | 3.27 | 4.1% |
| Sample 3 | 453 | 479 | 435 | 456 | 455 | 15.64 | 3.4% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0926-CAP|96-well plate precoated with anti- human CD244 antibody|1
EK0926-ST|Lyophilized recombinant human CD244 standard|10ng/tube×2
EK0926-DA|Biotinylated anti- human CD244 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
