Description
Boster’s human Aggrecan ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Aggrecan has been precoated onto 96-well plates. Standards (NSO, V20-G675) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Aggrecan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Aggrecan amount of sample captured in plate.
Synonyms: ACAN|AGC 1|AGC1|AGCAN|Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122)|Aggrecan 1|Aggrecan core protein|Aggrecan proteoglycan|Aggrecan structural proteoglycan of cartilage|Aggrecan1| ATEGQV|Cartilage specific proteoglycan core protein|Chondroitin sulfate proteoglycan 1|Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122|Chondroitin sulfate proteoglycan core protein 1|CSPG 1| CSPG1|CSPGCP|JSCATE|Large aggregating proteoglycan|mcspg|mgsk16|MSK 16|MSK16|SEDK
Species: Human Aggrecan
Assay: 1.56ng/ml-100ng/ml
Sensitivy: <50pg/ml Specifity: Natural and recombinant human Aggrecan
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated monoclonal antibody from mouse
Anticoagulant: /
Immunogen: NSO, V20-G675
Gene name: Aggrecan core protein / aggrecan
Protein name: Aggrecan core protein
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|3084|13064|43102|3322|13982|40208
Standard deviation|240.55|679.32|1939.59|275.72|908.83|1809.36
CV(%)|7.8%|5.2%|4.5%|8.3%|6.5%|4.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 3084 | 3011 | 3191 | 2954 | 3060 | 88.56 | 2.8% |
| Sample 2 | 13064 | 11683 | 11338 | 13036 | 12280 | 779.41 | 6.3% |
| Sample 3 | 43102 | 43347 | 44289 | 42847 | 43396 | 544.9 | 1.2% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0909-CAP|96-well plate precoated with anti- human Aggrecan antibody|1
EK0909-ST0|Lyophilized recombinant human Aggrecan standard|100ng/tube×2
EK0909-DA|Biotinylated anti- human Aggrecan antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
