Description
Boster’s human KIM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for KIM1 has been precoated onto 96-well plates. Standards(NSO, S21-T288) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KIM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human KIM1 amount of sample captured in plate.
Synonyms: HAVCR|_x000D_HAVcr-1|_x000D_Havcr1|_x000D__x000D__x000D_HAVR1_HUMAN|_x000D_Hepatitis A virus cellular receptor 1|_x000D_Kidney injury molecule 1|_x000D_KIM-1|_x000D_T cell immunoglobin domain and mucin domain protein 1|_x000D_T-cell immunoglobulin and mucin domain-containing protein 1|_x000D_T-cell membrane protein 1|_x000D_TIM|_x000D_TIM-1|_x000D_TIM1|_x000D_TIMD-1|_x000D_TIMD1
Species: Human KIM1
Assay: 31.2pg/ml-2000pg/ml
Sensitivy: <2pg/ml Specifity: Natural and recombinant human KIM1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin or EDTA
Immunogen: NSO, S21-T288
Gene name: Hepatitis A virus cellular receptor 1 / hepatitis A virus cellular receptor 1
Protein name: Hepatitis A virus cellular receptor 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|92|215|880|93|208|802
Standard deviation|5.42|9.24|39.6|5.48|11.02|40.1
CV(%)|5.9%|4.3%|4.5%|5.9%|5.3%|5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 92 | 93 | 93 | 91 | 92 | 0.82 | 0.8% |
| Sample 2 | 215 | 235 | 212 | 211 | 218 | 9.78 | 4.4% |
| Sample 3 | 880 | 798 | 777 | 920 | 843 | 58.47 | 6.9% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0883-CAP|96-well plate precoated with anti- human KIM1 antibody|1
EK0883-ST|Lyophilized recombinant human KIM1 standard|10ng/tube×2
EK0883-DA|Biotinylated anti- human KIM1 antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
