Description
Boster’s human PAI-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PAI-1 has been precoated onto 96-well plates. Standards(sf21, S22-P402) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PAI-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PAI-1 amount of sample captured in plate.
Synonyms: Endothelial plasminogen activator inhibitor|_x000D_PAI|_x000D_PAI-1|_x000D__x000D__x000D_PAI1|_x000D_PAI1_HUMAN|_x000D_PLANH1|_x000D_Plasminogen activator inhibitor 1|_x000D_Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1|_x000D_Serpin E1|_x000D_Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1|_x000D_SERPINE1
Species: Human PAI-1
Assay: 156pg/ml-10000pg/ml
Sensitivy: <10pg/ml Specifity: Natural and recombinant human PAI-1
Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
Antibodies: a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
Anticoagulant: heparin, EDTA or citrate
Immunogen: sf21, S22-P402
Gene name: Plasminogen activator inhibitor 1 / serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Protein name: Plasminogen activator inhibitor 1
Storage: Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|203|1208|5496|198|1244|5980
Standard deviation|12.38|71.27|423.19|15.04|95.78|520.26
CV(%)|6.1%|5.9%|7.7%|7.6%|7.7%|8.7%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 203 | 218 | 218 | 234 | 218 | 10.96 | 5% |
| Sample 2 | 1208 | 1308 | 1324 | 1403 | 1310 | 69.37 | 5.2% |
| Sample 3 | 5496 | 5184 | 6077 | 5696 | 5613 | 324 | 5.7% |
*number of samples for each test n=16.
Catalog number|Description|Quantity
EK0859-CAP|96-well plate precoated with anti- human PAI-1 antibody|1
EK0859-ST|Lyophilized recombinant human PAI-1 standard|10ng/tube×2
EK0859-DA|Biotinylated anti- human PAI-1antibody|130μl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130μl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
PLA-SEA|Adhesive cover|4
